3ohg

Protein Summary

Pfam update: This protein is a member of the DUF2233 (PF09992) family, described as putative periplasmic proteins  with unknown function and structure. This family is broadly distributed in Bacteria and Eukaryots, but not in Archea. Its mammalian homologs seem to have solid experimental based function of N-acetylglucosamine-1-phosphodiester �dc03-N-acetylglucosaminidase (“uncovering enzyme” or UCE or “Nagpa”), which is involved in converting mannose 6-phosphate (Man-6-P) diesters to Man-6-P monoesters [Ref]. Apparently based on this annotation, JGI calls proteins from this family "Exopolysaccharide biosynthesis proteins" but as far as I can tell no bacterial homolog have been directly studied.

This protein appears to be a lipoprotein as it has a predicted SpII cleavage site.

It has the Uniprot id A7LRK2 (A7LRK2_BACOV) and has been assigned to Pfam PF09992/DUF2233.

It is annotated as a protein of unknown function. According to Pfam, there are 494 unique proteins in this family from 184 species that contain the DUF2233 domain as part of proteins up to ~1000 residues and they are observed in 34 architectures.

It is present as a monomer in the crystal structure. PISA analysis suggests that a monomer is the preferred oligomeric form in solution.

FFAS did not yield any structural prediction for this family.

A search against the Conserved Domain Database produces hits to EpsL and DUF2233 family and PSI-BLAST shows hits to some proteins annotated as N-acetylmuramoyl-L-alanine amidase (AmiD).

Through a FATCAT search, the N-terminal domain has some similarity to human latexin (PDB id 2bo9) that is the endogenous inhibitor of metallocarboxypeptidase.

A DALI search results in numerous hits with Z-score > 4.5, for example with PDB ids 2obd (cholesteryl ester transfer protein), 2p4b (sigma E regulatory protein RSEB, and 2qfm (spermine synthase). The hits match to some portions of this target.

Further analysis has been performed.

A manuscript is almost complete on this structure and the human homolog.

Ligand Summary