3m7a

Protein Summary

Gene Saro_0823 from Novosphingobium aromaticivorans dsm 12444 translates into the YP_496102 protein, a member of the DUF192 group (PF02643), a very large (>500 homologs) family of mostly bacterial proteins, but also with homologs in several Plasmodium species. Saro_0823 is a homolog of Thermotoga maritima TM1668. The homolog in Caulobacter crescentus (CC1388) is associated with CspD, a cold shock protein (CC1387). However, the genomic context of Saro_0823 is most conserved with a putative xylose isomerase, suggesting a possible role in extracellular sugar processing. Saro_0821 is annotated as an AMP-dependent synthetase and ligase.

3m7a structure corresponds to the C-terminal (27-165) fragment of the YP_496102 protein. It is structurally rather unique as the best hit from Dali have a Z-score of  3.8 (1nt0, 2j1t, 3kq4) and it is a likely candidate for a new fold. Interestingly, many of the top Dali hits are involved in sugar metabolism. There are no obvious active site like cavities on the protein surface of 3m7a.

Note: The NCBI sequence (gi|87198845) seems to be shorter than JCSG PSCA sequence, which came from a preliminary assembly.  There is a similar structure solved by JCSG.

Fig. 1 monomer of 3m7a

Fig. 2 surface conservation in 3m7a structure

Fig. 3 sequence alignment of top blast hits fpr Saro_0823 amino acid sequence

Ligand Summary