3e11

Protein Summary

Gene Acel_2062 from Acidothermus cellulolyticus 11b codes for the YP_873820.1 protein, a member of the PFAM DUF1025 family (PF06262), a large (>200 members) family of hypothetical proteins from bacteria and human, soil and ocean metagenomes. The first structure from this family (Pfam e-value 0.1) was 2ejq from Thermus thermophilus HB8. Both structures have significant stuctural similarity to metalloproteases such as MMP16 (1rn8, Z=4) and others from Peptidase MA clan. Depite the low sequence identity (~8% sequence id), the classical MMP motif (HEXXHXXGXXH) is conserved in this family except for the last histidine. At the same time distant homology prediction programs recognize this similarity, even that with a marginal statistical significance (FFAS Z-score of -8.9 as compared to -9.5 significance treshold), suggesting that DUF1025 family is distantly related to metalloproteases and should be added to the Peptidase MA clan.

Structures of both representatives of DUF1025 family look like minimal versions of the metalloprotease fold, only three (out of 5) strands in the main beta sheet are conserved, loops are much closer and the overall length of all members of this family (~110 aa) is significantly shorter that the average MMP (~160aa)

SCOP classifies 3e11 (2ejq and 1xm5 too) in the alpha+beta class, zincin-like fold, metalloprotease catalytic domain superfamily, TTHA0227-like family. DALI hits are with 2ejq (Z=9), and the putative metal dependent hydrolase ybeY protein 1xm5 (Z=7) from PF02130.

Ligand Summary