| Title | Crystal structure of putative carboxylesterase (NP_786266.1) from Lactobacillus plantarum at 1.70 A resolution. | | Site | JCSG | | PDB Id | | Target Id | 379483 | | Molecular Characteristics | | Source | Lactobacillus plantarum | | Alias Ids | NP_786266.1 | Molecular Weight | 30416.98 Da. | | Residues | 276 | Isoelectric Point | 6.33 | | Sequence | mqveqrtlntaahpfqitaywldqisdfetavdypimiicpgggftyhsgreeapiatrmmaagmhtvvl nyqlivgdqsvypwalqqlgatidwittqasahhvdcqriilagfsagghvvatyngvatqpelrtryh ldhyqgqhaaiilgypvidltagfpttsaarnqittdarlwaaqrlvtpaskpafvwqtatdesvppin slkyvqamlqhqvatayhlfgsgihglalanhvtqkpgkdkylndqaaiwpqlalrwlqeqgllagny | | | BLAST FFAS |
| Structure Determination | | Method | XRAY | Chains | 2 | | Resolution (Å) | 1.70 | Rfree | 0.197 | | Matthews' coefficent | 2.30 | Rfactor | 0.166 | | Waters | 383 | Solvent Content | 46.49 |
| Ligand Information | | Ligands | EPE (4-(2-HYDROXYETHYL)-1-PIPERAZINE) x 2;PGR (R-1,2-PROPANEDIOL) x 3;PGO (S-1,2-PROPANEDIOL) x 4 | | Metals | CL (CHLORIDE) x 1 | |  | /content/body/table/tbody/tr/td[2]/center/table/tbody/tr[2]/td/center/span, reference to undefined name 'jmol': line 1, column 1 (click for details)MindTouch.Deki.Script.DekiScriptUndefinedNameException: reference to undefined name 'jmol': line 1, column 1
at MindTouch.Deki.Script.DekiScriptVar.Evaluate (MindTouch.Deki.Script.DekiScriptEnv env) [0x00000]
at MindTouch.Deki.Script.DekiScriptAccess.Evaluate (MindTouch.Deki.Script.DekiScriptEnv env, Boolean evaluateProperties) [0x00000]
at MindTouch.Deki.Script.DekiScriptAccess.Evaluate (MindTouch.Deki.Script.DekiScriptEnv env) [0x00000]
at MindTouch.Deki.Script.DekiScriptCall.Evaluate (MindTouch.Deki.Script.DekiScriptEnv env) [0x00000]
at MindTouch.Deki.Script.Dom.DekiScriptDomExpr.Evaluate (MindTouch.Deki.Script.DekiScriptEvalContext context, System.Xml.XmlNode parent, MindTouch.Deki.Script.DekiScriptEnv env) [0x00000] | |
| /content/body/table/tbody/tr/td[2]/center/table/tbody/tr[6]/td/center/table/tbody/tr/td/span, function 'template' failed (click for details)MindTouch.Deki.Script.DekiScriptInvokeException: function 'template' failed ---> System.ArgumentException: could not convert parameter 'target' (index 2) from list to str
at MindTouch.Deki.DekiScriptFunction+DekiScriptInvokeDelegateWrapper.Invoke (MindTouch.Deki.Script.DekiScriptLiteral arguments) [0x00000]
at MindTouch.Deki.DekiExtNativeFunction.Invoke (MindTouch.Deki.Script.DekiScriptLiteral arguments) [0x00000]
at MindTouch.Deki.Script.DekiScriptRuntime+<Invoke>d__0.MoveNext () [0x00000]
--- End of inner exception stack trace ---
at MindTouch.Deki.Script.DekiScriptRuntime+<Invoke>d__0.MoveNext () [0x00000]
at MindTouch.Tasking.Coroutine.Invoke (System.Func`1 invocation) [0x00000]
--- End of exception stack trace ---
at MindTouch.Deki.Script.DekiScriptRuntime.Invoke(XUri uri, DekiScriptLiteral args, DekiScriptEnv env, Result`1 result)
at MindTouch.Deki.Script.DekiScriptRuntime.Invoke(XUri uri, DekiScriptLiteral args, DekiScriptEnv env, Result`1 result)
at MindTouch.Deki.DekiWikiService.GetPageContents(DreamContext context, DreamMessage request, Result`1 response)
--- End of coroutine stack trace --- | | |
Protein Summary
The lp_2923 gene from Lactobacillus plantarum encodes a putative carboxylesterase from Lactobacillus plantarum.The protein shows sequence homology to members of Pfam PF07859 as determined by FFAS. Dali search results can find several similar structures such as 3BJR (NP_784706.1), 1JKM, 2HU7, 1JFR. Based on interface interaction calculation, this target may be a dimer.
lp_2923 has been directly compared with 379491, which is also from Lactobacillus plantarum. Both structures adopt a similar ?/?-hydrolase fold. In the structure of NP_784706.1, the conserved catalytic triad, Ser 113, Asp197 and His229, can be identified easily via direct structural comparison with other homologs. The TCOFFEE alignment between 379483 and 379491 indicates that these two targets have an almost identical sequence. Correspondingly, Ser 116, Asp 201 and His 233 in 379483 should be the conserved catalytic triad, as confirmed by direct structural comparison.
Ligand Summary
References
