3f40

Protein Summary

YP_677363.1 is a protein with 113  residues from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469), which is an abundant aerobic cellulolytic soil bacterium.  C. hutchinsonii is a gram-negative bacteriu that belongs to the phylum Bacteroidetes (also known as the Cytophaga-Flavobacterium-Bacteroides group). C. hutchinsonii utilizes very few substrates as sole carbon and energy sources.  Besides cellulose, its only known substrates are cellobiose and glucose. The sequence alignment results from NCBI can not provide a clear clue for function annotation.  The sequence search in Pfam suggests that this target (residue 9-111) should belong to the SnoaL-like polyketide cyclase (Pfam 07366).  There is one YP_677363.1molecule in each asymmetric unit.  The interface interaction suggests that the biomolecule of YP_677363.1be a dimer.

Figure 1. The YP_677363.1 molecule consists of α/β barrel mainly. 

Figure 2. The biomolecule of YP_677363.1 is a dimer based on interface interaction.

Figure 3. Protein YP_677363.1 (green) is structural homolog to 1SJW, 1TUH, 1VZZ and 2BGN. 

References

1.    Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mantsala P, Schneider G; , EMBO J 2004;23:1911-1921.: Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation. PUBMED:15071504 

2.    Xie, G., Bruce, D.C., Challacombe, J.F., Chertkov, O., Detter, J.C., Gilna, P., Han, C.S., Lucas, S., Misra, M., Myers, G.L., Richardson, P., Tapia, R., Thayer, N., Thompson, L.S., Brettin, T.S., Henrissat, B., Wilson, D.B., and McBride, M.J. "Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Appl. Environ. Microbiol. (2007) 73:3536-3546. 

3.    Robinson, A.,  Wu, P.S.-C.,  Harrop, S.J.,  Schaeffer, P.M.,  Dosztanyi, Z.,  Gillings, M.R.,  Holmes, A.J.,  Nevalainen, K.M.H.,  Stokes, H.W.,  Otting, G.,  Dixon, N.E.,  Curmi, P.M.G.,  Mabbutt, B.C.  (2005) Integron-associated Mobile Gene Cassettes Code for Folded Proteins: The Structure of Bal32a, a New Member of the Adaptable alpha+beta Barrel Family  J.Mol.Biol.   346: 1229-1241  (PDB 1TUH)

4.    Sultana, A.,  Kallio, P.,  Jansson, A.,  Wang, J.S.,  Niemi, J.,  Schneider, G.   (2004) Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation.  Embo J.   23: 1911-1921   (PDB: 1SJW)

5.    Johansson, P.,  Unge, T.,  Cronin, A.,  Arand, M.,  Bergfors, T.,  Jones, T.A.,  Mowbray, S.L.   (2005) Structure of an atypical epoxide hydrolase from Mycobacterium tuberculosis gives insights into its function.  J.Mol.Biol.   351: 1048-1056   (PDB: 2BNG)

6.    Jang, D.S.,  Cha, H.J.,  Cha, S.S.,  Hong, B.H.,  Ha, N.C.,  Lee, J.Y.,  Oh, B.H.,  Lee, H.S.,  Choi, K.Y.  (2004) Structural double-mutant cycle analysis of a hydrogen bond network in ketosteroid isomerase from Pseudomonas putida biotype B.  Biochem.J.   382: 967-973  (PDB 1VZZ)

Ligand Summary