| Title | Crystal structure of ethanolamine ammonia-lyase heavy chain (YP_013784.1) from Listeria monocytogenes 4b F2365 at 2.15 A resolution. To be published | | Site | JCSG | | PDB Id | | Target Id | 366145 | | Molecular Characteristics | | Source | Listeria monocytogenes str. 4b f2365 | | Alias Ids | TPS1472,YP_013784.1 | Molecular Weight | 49863.95 Da. | | Residues | 454 | Isoelectric Point | 5.20 | | Sequence | milktnlfghtyqfksitdvlakaneeksgdrlagvaaesaeervaakvvlskmtlgdlrnnpvvpyet devtriiqdqvndrihdsiknwtveelrewildhkttdadikrvargltseiiaavtklmsnldliyga kkirviahanttiglpgtfsarlqpnhptddpdgilaslmegltygigdaviglnpvddstdsvvrlln kfeefrskwdvptqtcvlahvktqmeamrrgaptglvfqsiagsekgntafgfdgatieearqlalqsg aatgpnvmyfetgqgselssdahfgvdqvtmearcygfakkfdpflvntvvgfigpeylydskqvirag ledhfmgkltgismgcdvcytnhmkadqndvenlsvlltaagcnfimgiphgddvmlnyqttgyhetat lrelfglkpikefdqwmekmgfsengkltsragdasiflk | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 6 | | Resolution (Å) | 2.15 | Rfree | 0.279 | | Matthews' coefficent | 2.28 | Rfactor | 0.228 | | Waters | 754 | Solvent Content | 46.12 |
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The gene LMOf2365_1185 (also known as eutB) from Listeria monocytogenes 4b f2365 encodes ethanolamine ammonia-lyase large (alpha) subunit (EutB) PF06751 COG4303. The protein belongs to the class of alpha and beta (a+b) proteins and adopts a TIM beta/alpha-barrel fold type SCOP51350. Ethanolamine ammonia-lyase (alternative name: ethanolamine deaminase) EC:4.3.1.7 is the enzyme which catalyzes the following reaction: ethanolamine <=> acetaldehyde + NH(3). It requires cobalamin as a cofactor. The enzyme enables bacteria to survive on small organic molecules such as ethanolamine as the sole source for carbon and nitrogen.
Ligand Summary
References
