| Title | Crystal structure of Geranyltranstransferase (EC 2.5.1.10) (tm0161) from THERMOTOGA MARITIMA at 1.90 A resolution. | | Site | JCSG | | PDB Id | | Target Id | 282041 | | Molecular Characteristics | | Source | Thermotoga maritima | | Alias Ids | TM0161 | Molecular Weight | 31022.42 Da. | | Residues | 272 | Isoelectric Point | 5.41 | | Sequence | mkkekveerireilrpgwdllteeamlysatvggkrirpllvltlgedlgveeeklldvavavelfhtas lihddlppidnadfrrgkpschrtygediallagdglfflafsqiskignskifeefsetayklllgea mdveferrkmevsqemvermyafktgalfafcfsapfilkgkdhtkmkllgekfgvafqiyddlkdilg sfekvgkdlgkdtekvtlvkkvgiqkaremadkyyeevlkgieseglfrtlfllkelkqmveer | | | BLAST FFAS |
| Structure Determination | | Method | XRAY | Chains | 1 | | Resolution (Å) | 1.90 | Rfree | 0.203 | | Matthews' coefficent | 3.32 | Rfactor | 0.169 | | Waters | 171 | Solvent Content | 62.67 |
| Pathway | | Reactions found in Metabolic Reconstruction: TM0161 | Metabolic Subsystem: Terpenoid biosynthesis
Reaction: :
dmpp
Name:Dimethylallyl diphosphate
Formula:C5H9O7P2
KEGG:
C00235
+
ipdp
Name:Isopentenyl diphosphate
Formula:C5H9O7P2
KEGG:
C00129
-->
grdp
Name:Geranyl diphosphate
Formula:C10H17O7P2
KEGG:
C00341
+
ppi
Name:Diphosphate
Formula:HO7P2
KEGG:
C00013
Name: dimethylallyltranstransferase
Classification: EC:2.5.1.1
| Metabolic Subsystem: Terpenoid biosynthesis
Reaction: :
grdp
Name:Geranyl diphosphate
Formula:C10H17O7P2
KEGG:
C00341
+
ipdp
Name:Isopentenyl diphosphate
Formula:C5H9O7P2
KEGG:
C00129
-->
frdp
Name:Farnesyl diphosphate
Formula:C15H25O7P2
KEGG:
C00448
+
ppi
Name:Diphosphate
Formula:HO7P2
KEGG:
C00013
Name: geranyltranstransferase
Classification: EC:2.5.1.10
|
| Ligand Information | | Ligands | UNL (UNKNOWN) x 2;EDO (1,2-ETHANEDIOL) x 10 | | Metals | | | |
Protein Summary
The TM0161 gene of Thermotoga maritima encodes a geranyltranstransferase (EC 1.5.1.10, PF00348, COG0142). The TM0161 structure is an all-alpha protein with a terpenoid synthase fold and is very similar (backbone rmsd of 1.7 Å over 254 residues with a sequence identity of 31%) to the geranyltranstransferase from Shigella flexneri (PDB id: 2for). The structural similarity also extends to orthologs from Agrobacterium tumefaciens (PDB id: 2h8o), Escherichia coli (PDB ids: 1rqj, 1rtr, 1rqi), and other hyperthermophiles such as Pyrococcus horikoshii (PDB id: 1wy0) and Thermus thermophilus (PDB id: 1wmw). More details on the structure and reaction mechanism can be found in Hosfield 2004, Kloer 2006, K-M Chen 2008.
Ligand Summary
References
