| Title | Crystal structure of Endoglucanase (TM1752) from Thermotoga maritima at 2.05 A resolution. To be published | | Site | JCSG | | PDB Id | | Target Id | 359798 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1435,TM1752 | Molecular Weight | 39298.98 Da. | | Residues | 329 | Isoelectric Point | 5.88 | | Sequence | mnntiprwrgfnlleafsikstgnfkeedflwmaqwdfnfvripmchllwsdrgnpfiiredffekidr vifwgekygihicislhrapgysvnkeveektnlwkdetaqeafihhwsfiarrykgissthlsfnlin eppfpdpqimsvedhnslikrtiteirkidperliiidglgygnipvddltientvqscrgyipfsvth ykaewvdskdfpvpewpngwhfgeywnrekllehyltwiklrqkgievfcgemgaynktphdvvlkwle dlleifktlnigfalwnfrgpfgildserkdveyeewyghkldrkmlellrky | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 1 | | Resolution (Å) | 2.05 | Rfree | 0.21244 | | Matthews' coefficent | 2.53 | Rfactor | 0.16804 | | Waters | 212 | Solvent Content | 51.06 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM1752 | Name: hydrolysis of galactomannan
Other genes that carryout this rxn:TM1751 TM1624 TM1192
Metabolic Subsystem: Mannan Metabolism
Reaction: :
galman6
Name:"galactomannan(n=6 repeat units mannose, beta-1,4 man)"
Formula:C36H62O31
KEGG:
+
h2o
Name:H2O
Formula:H2O
KEGG:
C00001
-->
gal
Name:D-Galactose
Formula:C6H12O6
KEGG:
C00124
+
man
Name:D-Mannose
Formula:C6H12O6
KEGG:
C00159
Classification: EC:3.2.1.4
| Name: hydrolysis of glucomannan
Other genes that carryout this rxn:TM1751 TM1624
Metabolic Subsystem: Mannan Metabolism
Reaction: :
glcman4
Name:"glucomannan (n=4 repeat units, glc beta-1,4 man)"
Formula:C24H42O21
KEGG:
+
h2o
Name:H2O
Formula:H2O
KEGG:
C00001
-->
glc-D
Name:D-Glucose
Formula:C6H12O6
KEGG:
C00031
+
man
Name:D-Mannose
Formula:C6H12O6
KEGG:
C00159
Classification: EC:3.2.1.4
| Name: hydrolysis of glucomannan
Other genes that carryout this rxn:TM1751 TM1624
Metabolic Subsystem: Mannan Metabolism
Reaction: :
glcman6
Name:"glucomannan (n=6 repeat units, glc beta-1,4 man)"
Formula:C36H62O31
KEGG:
+
h2o
Name:H2O
Formula:H2O
KEGG:
C00001
-->
glc-D
Name:D-Glucose
Formula:C6H12O6
KEGG:
C00031
+
man
Name:D-Mannose
Formula:C6H12O6
KEGG:
C00159
Classification: EC:3.2.1.4
| Name: hydrolysis of galactomannan
Other genes that carryout this rxn:TM1751 TM1624 TM1192
Metabolic Subsystem: Mannan Metabolism
Reaction: :
galman4
Name:"galactomannan(n=4 repeat units mannose, beta-1,4 man)"
Formula:C24H42O21
KEGG:
+
h2o
Name:H2O
Formula:H2O
KEGG:
C00001
-->
gal
Name:D-Galactose
Formula:C6H12O6
KEGG:
C00124
+
man
Name:D-Mannose
Formula:C6H12O6
KEGG:
C00159
Classification: EC:3.2.1.4
|
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The gene TM1752 from Thermotoga maritima encodes the enzyme endoglucanase PF00150 EC:3.2.1.4 COG4305. Accepted name: cellulase; alternative names: celludextrinase, endo-1,4-beta-D-glucanase. The enzyme belongs to the family of alpha and beta (a+b) proteins and reveals TIM beta/alpha-barrel fold type SCOP51350. The enzyme breaks down cellulose to beta-glucose. This type of cellulase is produced mainly by symbiotic bacteria in the ruminating chambers of herbivores. The structures of several enzyme homologues from different organisms have been solved: 1CEN, Clostridium thermocellum.
Ligand Summary
References
