| Title | Crystal structure of Alpha-glucosidase (TM0752) from Thermotoga maritima at 2.50 A resolution. To be published | | Site | JCSG | | PDB Id | | Target Id | 282622 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1231,TM0752 | Molecular Weight | 55354.60 Da. | | Residues | 471 | Isoelectric Point | 5.85 | | Sequence | mkisiigagsvrfalqlvgdiaqteelsredthiymmdvherrlnasyilarkyveelnspvkivktss ldeaidgadfiintaypydpryhdsgsqrwdevtkvgekhgyyrgidsqelnmvstytyvlssypdmkl aleiaekmkkmapkaylmqtanpvfeitqavrrwtganivgfchgvagvyevfekldldpeevdwqvag vnhgiwlnrfryrgedayplldewiekklpewepknpwdtqmspaamdmykfygmlpigdtvrngswky hynletkkkwfgkfggidneverpkfheqlrrarerliklaeevqqnpgmklteehpeifpkgklsgeq hipfinaiannkrvrlflnvenqgtlkdfpddvvmelpvwvdccgihrekvepdlthrikifylwpril rmewnleayisrdrkvleeilirdprtksyeqivqvldeifnlpfneelrryykekl | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 1 | | Resolution (Å) | 2.50 | Rfree | 0.25602 | | Matthews' coefficent | 2.38 | Rfactor | 0.1944 | | Waters | 48 | Solvent Content | 47.87 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM0752 | Name: sucrose hydrolyzing enzyme
Other genes that carryout this rxn: TM1068 TM1414
Metabolic Subsystem: Sucrose Metabolism
Reaction: :
h2o
Name:H2O
Formula:H2O
KEGG:
C00001
+
sucr
Name:Sucrose
Formula:C12H22O11
KEGG:
C00089
-->
fru
Name:D-Fructose
Formula:C6H12O6
KEGG:
C00095
+
glc-D
Name:D-Glucose
Formula:C6H12O6
KEGG:
C00031
Classification: EC:3.2.1.26
|
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The TM0752 gene from Thermotoga maritima encodes a 6-phospho-alpha-glycosidase belonging to the family 4 glycosyl hydrolases PF02056 COG0366. Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. Glycoside hydrolase family 4 comprises enzymes with several known activities: 6-phospho-beta-glucosidases, 6-phospho-alpha-glucosidase, alpha-galactosidases. 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn2+, Fe2+, Co2+, or Ni2+) for activity [Ref]. Thus, the TM0752 codes for NAD(H)-dependent 6-phospho-alpha-glycosidase. The crystal structure of a possible isoform of this enzyme from the same organism has been reported (1OBB).
Ligand Summary
References
