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The Open Protein Structure Annotation Network
PDB Keyword
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1sj5

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171. Protein Sci. 13 3187-3199 2004
    Site JCSG
    PDB Id 1sj5 Target Id 358348
    Molecular Characteristics
    Source Thermotoga maritima msb8
    Alias Ids TPS1389,TM0160 Molecular Weight 16145.69 Da.
    Residues 145 Isoelectric Point 4.73
    Sequence mrkawvktlaldrvsntpvvilgiegtnrvlpiwigaceghalalamekmefprplthdlllsvlesle arvdkviihslkdntfyatlvirdltytdeedeeaalididsrpsdaiilavktgapifvsdnlvekhs ielevne
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 2
    Resolution (Å) 2.80 Rfree 0.27555
    Matthews' coefficent 2.26 Rfactor 0.21571
    Waters Solvent Content 45.55

    Ligand Information
    Ligands
    Metals

    Jmol

     
    Google Scholar output for 1sj5
    1. On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171
    G Spraggon, D Pantazatos, HE Klock, IA Wilson - Protein , 2004 - Wiley Online Library
     
    2. Structural and biochemical investigation of the role in proofreading of a _ hairpin loop found in the exonuclease domain of a replicative DNA polymerase of the B
    M Hogg, P Aller, W Konigsberg, SS Wallace - Journal of Biological , 2007 - ASBMB
     

    Protein Summary

    The gene TM0160 from Thermotoga maritima encodes the NP_227975 protein of unknown function DUF151 PF02577. Analysis of TM0160 genome vicinity finds a possible functional partner based on shared neigborhood (score 0.87) with a geranyltrans-transferase (TM0161).

    SCOP classifies 1sj5 in the alpha+beta class, TM0160 fold and family. DALI finds no significant hits (Z<3).

    A BLAST sequence alignment includes the C-terminal domain of the UvrB/UvrC protein from Polaribacter sp. 2FDC with an E-value=3e-38, 31% amino acid sequence identity  and 7% gaps.  UvrB is the central component in the prokaryotic nucleotide excision repair system.

    Additionally, DUF151 can be related structurally to the family of response regulators REC, like CheY protein ( 3F7N).  The response regulators act as phosphorylation-activated switches that affect a cellular response, usually by transcriptional regulation. Most of these proteins consist of two domains, an N-terminal response regulator receiver domain, and a variable C-terminal effector domain with DNA-binding activity.  Thus, temptatively DUF151 could be a C-terminal effector domain with DNA-binding activity of either DNA repair enzyme or a REC-like protein.

     

    1SJ5 is the N-terminal fragment of 1VJL structure.

    Ligand Summary



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