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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of the C-terminal fragment of the putative flagellar motor switch protein FliN (TM0680a) from Thermotoga maritima at 1.85 A resolution. To be published
    Site JCSG
    PDB Id 1o6a Target Id 356088
    Molecular Characteristics
    Source Thermotoga maritima msb8
    Alias Ids TPS1355,TM0680 Molecular Weight 17302.83 Da.
    Residues 162 Isoelectric Point 4.16
    Sequence mteneflsqeeidkllsdsdtggvltpeekdmigeigniamgsaattlsmilgrdihitvptvreekmk nvksdfsgeqvvvsveytegleglnvlvldkklvaviadlmmggsgeveteeldeiklsavgeamnqmm gsaatslsellgitinisppkvei
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 1.85 Rfree 0.21199
    Matthews' coefficent 2.50 Rfactor 0.17102
    Waters 150 Solvent Content 50.33

    Ligand Information


    Google Scholar output for 1o6a
    1. The Buccaneer software for automated model building. 1. Tracing protein chains
    K Cowtan - Acta Crystallographica Section D: Biological , 2006 - scripts.iucr.org
    2. Conserved features of type III secretion
    AP Tampakaki, VE Fadouloglou, AD Gazi - Cellular , 2004 - Wiley Online Library
    3. Crystal structure of the flagellar rotor protein FliN from Thermotoga maritima
    PN Brown, MAA Mathews, LA Joss, CP Hill - Journal of , 2005 - Am Soc Microbiol
    4. Structure and function of an unusual family of protein phosphatases: the bacterial chemotaxis proteins CheC and CheX
    SY Park, X Chao, G Gonzalez-Bonet, BD Beel - Molecular cell, 2004 - Elsevier
    5. Structural and functional studies of the enteropathogenic Escherichia coli type III needle complex protein EscJ
    VF Crepin, S Prasannan, RK Shaw - Molecular , 2005 - Wiley Online Library
    6. Organization of FliN subunits in the flagellar motor of Escherichia coli
    K Paul, DF Blair - Journal of bacteriology, 2006 - Am Soc Microbiol
    7. Mutational analysis of the flagellar rotor protein FliN: identification of surfaces important for flagellar assembly and switching
    K Paul, JG Harmon, DF Blair - Journal of bacteriology, 2006 - Am Soc Microbiol
    8. Bacterial nanomachines: the flagellum and type III injectisome
    M Erhardt, K Namba, KT Hughes - Cold Spring Harbor , 2010 - cshperspectives.net
    9. Shotgun crystallization strategy for structural genomics II: crystallization conditions that produce high resolution structures for T. maritima proteins
    R Page, AM Deacon, SA Lesley - Journal of structural and , 2005 - Springer
    10. On the quaternary association of the type III secretion system HrcQB-C protein: Experimental evidence differentiates among the various oligomerization models
    VE Fadouloglou, MN Bastaki, AE Ashcroft - Journal of structural , 2009 - Elsevier
    11. Single-Molecule Studies of Rotary Molecular Motors
    T Pilizota, Y Sowa, RM Berry - Handbook of Single-Molecule Biophysics, 2009 - Springer
    12. Molecular Assembly in Natural and Engineered Systems
    S Howorka - 2011 - books.google.com

    Protein Summary

    The gene TM0680 from Thermotoga maritima encodes the C-terminal domain of the flagellar motor switch protein FliY (PF07859).  The complete FliY protein possesses phosphatase activity [Ref].  Structurally, it can be related to CheC protein from the same organism 1XKR SCOP103038 PF04509.  The protein belongs to a group of factors controlling bacterial chemotaxis.

    Ligand Summary





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