| Title | Crystal structure of Uracil phosphoribosyltransferase (TM0721) from Thermotoga maritima at 2.30 A resolution. To be published | | Site | JCSG | | PDB Id | | Target Id | 282591 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1227,TM0721 | Molecular Weight | 23318.98 Da. | | Residues | 209 | Isoelectric Point | 5.62 | | Sequence | mknlvvvdhplikhkltimrdkntgpkefrellreitlllayeatrhlkceevevetpitktigyrind kdivvvpilraglvmadgilellpnasvghigiyrdpetlqaveyyaklpplnddkevflldpmlatgv ssikaieilkengakkitlvaliaapegveavekkyedvkiyvaalderlndhgyiipglgdagdrlfrtk | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 4 | | Resolution (Å) | 2.30 | Rfree | 0.23635 | | Matthews' coefficent | 2.44 | Rfactor | 0.16658 | | Waters | 355 | Solvent Content | 49.20 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM0721 | Name: uracil phosphoribosyltransferase
Metabolic Subsystem: Pyrimidine Metabolism
Reaction: :
prpp
Name:5-Phospho-alpha-D-ribose 1-diphosphate
Formula:C5H8O14P3
KEGG:
C00119
+
ura
Name:Uracil
Formula:C4H4N2O2
KEGG:
C00106
-->
ppi
Name:Diphosphate
Formula:HO7P2
KEGG:
C00013
+
ump
Name:UMP
Formula:C9H11N2O9P
KEGG:
C00105
Classification: EC:2.4.2.9
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| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The TM0721 gene from Thermotoga maritima encodes an uracil phosphoribosyltransferase (PF00156, COG0035, EC 2.4.2.9). The TM0721 structure adopts a phosphoribosyl-transferase-like fold and shows strong structural similarity with the homolog from another hyperthormophile, Thermus thermophilus (PDB id: 1v9s) with a main-chain rmsd of 1.0 Å over 206 residues and a sequence identity of 57%. Similar results were obtained with homologs from Escherichia coli (PDB id: 2ehj), Aquifex aeolicus (PDB id: 2e55), Burkholderia pseudomallei (PDB id: 3dmp) and Bacillus caldolyticus (PDB id: 1i5e). For more details on the structure and function of this enzyme see Kadziola 2002.
Ligand Summary
References
