| Title | Crystal structure of a formiminotetrahydrofolate cyclodeaminase (TM1560) from Thermotoga maritima at 2.80 A resolution reveals a new fold. Proteins 58 976-981 2005 | | Site | JCSG | | PDB Id | | Target Id | 283417 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1299,TM1560 | Molecular Weight | 22712.09 Da. | | Residues | 202 | Isoelectric Point | 4.96 | | Sequence | meverlslkefcdmvaerkptpgggavgsvvgamacalaemvanftrkkkgyedvepemeriveameea rlklfdlakkdmeafekvmkaykssegelqnalkeaasvpmdvirvmkdlaheleklaefgnknlasdt lnaadlchavfqvekvnvlinlkeisdetfrknmleeleeqeaqiegcyqrvkkmlegivwssk | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 2 | | Resolution (Å) | 2.80 | Rfree | 0.27814 | | Matthews' coefficent | 2.84 | Rfactor | 0.19927 | | Waters | | Solvent Content | 56.40 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM1560 | Name: formimidoyltransferase cyclodeaminase reversible
Metabolic Subsystem: Folate Metabolism
Reaction: :
5forthf
Name:5-Formiminotetrahydrofolate
Formula:C20H22N8O6
KEGG:
C00664
+
h
Name:H+
Formula:H
KEGG:
C00080
<==>
methf
Name:"5,10-Methenyltetrahydrofolate"
Formula:C20H20N7O6
KEGG:
C00445
+
nh4
Name:Ammonium
Formula:H4N
KEGG:
C01342
Classification: EC:4.3.1.4
|
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
Formiminotetrahydrofolate cyclodeaminase (TM1560) from
Thermotoga maritima (FTCD_CD, EC 4.3.1.4; COG3404, pfam04961) is involved in folate metabolism (PubMed:
15651027).
TM1560 is similar to protein domain of bifunctional, Golgi-associated formiminotransferase cyclodeaminase (PDB id: 1tt9) - a mammalian protein that plays important roles in coupling histidine catabolism with folate metabolism and integrating the Golgi complex with the vimentin intermediate filament cytoskeleton (PubMed:15272307).
TM1560 has methenyltetrahydrofolate cyclohydrolase-like fold (SCOP sunid:101261) - closed 5 helical bundle with left-handed twist.
Ligand Summary
References
