| Title | Crystal structure of O-acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.8 A resolution. Proteins 56 387-391 2004 | | Site | JCSG | | PDB Id | | Target Id | 282537 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1224,TM0665 | Molecular Weight | 31128.65 Da. | | Residues | 291 | Isoelectric Point | 8.92 | | Sequence | mmerligstpivrldsidsriflkleknnpggsvkdrpalfmildaekrgllkngiveptsgnmgiaia migakrghrviltmpetmsverrkvlkmlgaelvltpgelgmkgavekaleisretgahmlnqfenpyn vyshqfttgpeilkqmdyqidafvagvgtggtisgvgrvlkgffgngvkivavepakspvlsggqpgkh aiqgigagfvpkildrsvidevitvedeeayemarylakkegllvgissganvaaalkvaqklgpdarv vtvapdhaerylsil | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 2 | | Resolution (Å) | 1.80 | Rfree | 0.19807 | | Matthews' coefficent | 2.32 | Rfactor | 0.15738 | | Waters | 937 | Solvent Content | 46.50 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM0665 | Name: cysteine synthase
Other genes that carryout this rxn:TM0882
Metabolic Subsystem: Cysteine Metabolism
Reaction: :
acser
Name:O-Acetyl-L-serine
Formula:C5H9NO4
KEGG:
C00979
+
h2s
Name:Hydrogen sulfide
Formula:H2S
KEGG:
C00283
-->
ac
Name:Acetate
Formula:C2H3O2
KEGG:
C00033
+
cys-L
Name:L-Cysteine
Formula:C3H7NO2S
KEGG:
C00097
+
h
Name:H+
Formula:H
KEGG:
C00080
|
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The TM0665 gene of
Thermotoga maritima encodes a pyridoxal 5'-phosphate dependent O-acetylserine sulfhydrylase, that catalyzes the beta-replacement of the acetyl group in O-acetyl-L-serine
by sulfide to generate L-cysteine with production of
acetate (EC 4.2.99.8; CysK; COG0031, PubMed:
15211522). This enzyme can also generate S-sulfo-L-cysteine using thiosulfate instead of sulfide. Metazoa and fungi does not have proteins homologous to TM0665, where cysteine is derived from methionine, but not from serine as in bacteria, archaea, and plants.
Protein encoded by TM065 has tryptophan synthase beta subunit-like PLP-dependent enzymes fold (SCOP sunid: 53685) with two similar domains related by pseudo dyad. Each domain has 3 layers (a/b/a) core with parallel beta-sheet of 4 strands (order 3214). Please note, PDB structure 1j6n of TM0665 was replaced by PDB structure 1o58.
Ligand Summary
References
