| Title | Crystal structure of gamma-glutamyl phosphate reductase (TM0293) from Thermotoga maritima at 2.0 A resolution. Proteins 54 157-161 2004 | | Site | JCSG | | PDB Id | | Target Id | 282169 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1197,TM0293 | Molecular Weight | 46401.77 Da. | | Residues | 415 | Isoelectric Point | 5.98 | | Sequence | mdellekakkvreawdvlrnattreknkaikkiaeklderrkeileanridvekarergvkeslvdrla lndkridemikacetviglkdpvgevidswvredglriarvrvpigpigiiyesrpnvtvettilalks gntillrggsdalnsnkaivsairealketeipessvefientdrslvlemirlreylslviprggygl isfvrdnatvpvletgvgnchifvdesadlkkavpviinaktqrpgtcnaaekllvhekiakeflpviv eelrkhgvevrgcektreivpdvvpateddwpteyldliiaikvvknvdeaiehikkystghsesilte nysnakkfvseidaaavyvnastrftdggqfgfgaeigistqrfhargpvglrelttykfvvlgeyhvre | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 1 | | Resolution (Å) | 2.00 | Rfree | 0.203 | | Matthews' coefficent | 2.57 | Rfactor | 0.16 | | Waters | 299 | Solvent Content | 51.67 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM0293 | Name: glutamate-5-semialdehyde dehydrogenase
Metabolic Subsystem: Proline Biosynthesis
Reaction: :
glu5p
Name:L-Glutamate 5-phosphate
Formula:C5H8NO7P
KEGG:
C03287
+
h
Name:H+
Formula:H
KEGG:
C00080
+
nadph
Name:Nicotinamide adenine dinucleotide phosphate - reduced
Formula:C21H26N7O17P3
KEGG:
C00005
-->
glu5sa
Name:L-Glutamate 5-semialdehyde
Formula:C5H9NO3
KEGG:
C01165
+
nadp
Name:Nicotinamide adenine dinucleotide phosphate
Formula:C21H25N7O17P3
KEGG:
C00006
+
pi
Name:Phosphate
Formula:HO4P
KEGG:
C00009
Classification: EC:1.2.1.41
|
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The TM0293 gene of
Thermotoga maritima encodes gamma-glutamyl phosphate reductase (GPR; EC
1.2.1.41), an enzyme that catalyzes the second step of
proline biosynthesis, the reversible nicotinamide adenine
dinucleotide phosphate (NADPH)-dependent reduction of
L-glutamyl phosphate to phosphate and L-glutamate
5-semialdehyde (PubMed:
14705032).
Protein encoded by TM0293 gene has ALDH-like fold (SCOP sunid:53719) with two similar domains (each with 3 layers a/b/a sandwich) with parallel beta-sheet of 5 strands (order 32145).
Ligand Summary
References
