| Title | Crystal structure of a Deoxyribose-phosphate aldolase (TM_1559) from THERMOTOGA MARITIMA at 1.75 A resolution. To be published | | Site | JCSG | | PDB Id | | Target Id | 283416 | | Related PDB Ids 3r13 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1298,TM1559 | Molecular Weight | 27283.87 Da. | | Residues | 248 | Isoelectric Point | 5.24 | | Sequence | mieyrieeavakyrefyefkpvresagiedvksaiehtnlkpfatpddikklclearenrfhgvcvnpc yvklareelegtdvkvvtvvgfplganetrtkaheaifavesgadeidmvinvgmlkakeweyvyedir svvesvkgkvvkviietcyldteekiaacvisklagahfvktstgfgtggataedvhlmkwivgdemgv kasggirtfedavkmimygadrigtssgvkivqggeerygg | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 2 | | Resolution (Å) | 1.75 | Rfree | 0.1915 | | Matthews' coefficent | 2.02 | Rfactor | 0.1529 | | Waters | 256 | Solvent Content | 39.03 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM1559 | Name: deoxyribose-phosphate aldolase reversible
Metabolic Subsystem: Energy Metabolism
Reaction: :
2dr5p
Name:2-Deoxy-D-ribose 5-phosphate
Formula:C5H9O7P
KEGG:
C00673
<==>
acald
Name:Acetaldehyde
Formula:C2H4O
KEGG:
C00084
+
g3p
Name:Glyceraldehyde 3-phosphate
Formula:C3H5O6P
KEGG:
C00661
Classification: EC:4.1.2.4
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| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The gene TM1559 from Thermotoga maritima encodes 2-deoxyribose-5-phosphate aldolase (DERA) from the DeoC family of aldolase enzymes PF01791 COG0274. This enzyme participates in the pentose phosphate pathway. DERA belongs to the class I aldolases and catalyzes a reversible aldol reaction between acetaldehyde and glyceraldehyde 3-phosphate to generate 2-deoxyribose 5-phosphate. DERA is unique in catalyzing the aldol reaction between two aldehydes. Because of its unique enzymatic reaction and broad substrate specificity, the enzyme has become an industrial biocatalyst, offering an environmentally benign alternative to chiral transition metal catalysis of the asymmetric aldol reaction. The enzyme has been optimized for numerous industrial chemistry processes using the directed evolution approach [Ref].
Ligand Summary
References
