| Title | Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline. Proc.Natl.Acad.Sci.USA 99 11664-11669 2002 | | Site | JCSG | | PDB Id | | Target Id | 282296 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1203,TM0423 | Molecular Weight | 39689.39 Da. | | Residues | 364 | Isoelectric Point | 5.17 | | Sequence | mitttifpgryvqgagainileeelsrfgerafvviddfvdknvlgenffssftkvrvnkqifggecsd eeierlsglveeetdvvvgigggktldtakavayklkkpvvivptiastdapcsalsviytpngefkry lflprnpdvvlvdteivakaparflvagmgdalatwfeaesckqkyapnmtgrlgsmtayalarlcyet lleygvlakrsveeksvtpalekiveantllsglgfesgglaaahaihngltvlenthkylhgekvaig vlaslfltdkprkmieevysfceevglpttlaeigldgvsdedlmkvaekacdknetihnepqpvtskd vffalkaadrygrmrknlt | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 1 | | Resolution (Å) | 1.50 | Rfree | 0.189 | | Matthews' coefficent | 2.30 | Rfactor | 0.173 | | Waters | 227 | Solvent Content | 46.59 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM0423 | Name: Glycerol dehydrogenase reversible
Metabolic Subsystem: Alternate Carbon Metabolism
Reaction: :
glyc
Name:Glycerol
Formula:C3H8O3
KEGG:
C00116
+
nad
Name:Nicotinamide adenine dinucleotide
Formula:C21H26N7O14P2
KEGG:
C00003
<==>
dha
Name:Dihydroxyacetone
Formula:C3H6O3
KEGG:
C00184
+
h
Name:H+
Formula:H
KEGG:
C00080
+
nadh
Name:Nicotinamide adenine dinucleotide - reduced
Formula:C21H27N7O14P2
KEGG:
C00004
Classification: EC:1.1.1.6
|
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The TM0423 gene of Thermotoga maritima encodes a zinc-containing glycerol dehydrogenase (EC:1.1.1.6, PFAM:PF00465, COG:COG0371) that catalyzes the oxidation of glycerol to dihydroxyacetone with the simultaneous reduction of oxidized nicotinamide adenine dinucleatide (NAD+) to reduced nicotinamide adenine dinucleotide (NADH) (PubMed:12193646).
Enzyme similar to TM0423 is Fe-dependent and under anaerobic conditions is involved in dissimilation of glucose, and also has a protective role against oxidative stress in aerobic conditions (PMID:12783863).
The discovery of a coenzyme B12 independent glycerol dehydratase (similar to TM0423), from Clostridium butyricum should significantly influence the development of an economical vitamin B12-free biological process for the production of 1,3-propanediol (1,3-PD) from renewable resources (PMID:12704244).
Protein encoded by TM0423 has dehydroquinate synthase-like fold (SCOP sunid:56795) with 2 domains: 1st - alpha/beta of a Rossmann-fold topology that binds NAD, and 2nd - multihelical array. This protein belongs to protein family of iron-containing alcohol dehydrogenases (SCOP sunid:69892). TM0423 has both sequence (FFAS03) and structural (FATCAT) similarity to several structures (i.e.: PDB:1jpuA, PDB:1ta9A, PDB:2bi4A, PDB:1vljA, PDB:1oj7A, PDB:1dqsA, PDB:1xagA, and PDB:1ujnA).
Ligand Summary
References
