| Title | Crystal structure of Glucosamine-6-phosphate deaminase (TM0813) from Thermotoga maritima at 1.8 A resolution. To be published | | Site | JCSG | | PDB Id | | Target Id | 282683 | | Molecular Characteristics | | Source | Thermotoga maritima msb8 | | Alias Ids | TPS1235,TM0813 | Molecular Weight | 37369.23 Da. | | Residues | 330 | Isoelectric Point | 5.75 | | Sequence | msktlkeitdqknelkkffenfvlnlekteifseiqknltdevlfvgcgssynlaltisyyfervlkir tkaipagevafqkipdleerglaflfsrtgnttevllandvlkkrnhrtigitieeesrlakesdlplv fpvreeaivmtksfsmillslmfladkiagnsterfselvgyspeffdiswkviekidlkehdhfvflg mseffgvslesalkciemsltfseaystleyrhgpkalvkkgtlvfmqkvsgmdeqekrlrkeleslga tvlevgeggdipvsndwksaflrtvpaqilgyqkaisrgispdkpphlektvvl | | | BLAST FFAS | | Structure Determination | | Method | XRAY | Chains | 1 | | Resolution (Å) | 1.80 | Rfree | 0.231 | | Matthews' coefficent | 2.51 | Rfactor | 0.192 | | Waters | 294 | Solvent Content | 50.60 |
| Pathway | | Reactions found in Metabolic Reconstruction for TM0813 | Name: glucosamine-6-phosphate deaminase reversible
Metabolic Subsystem: Aminosugar Metabolism
Reaction: :
gam6p
Name:D-Glucosamine 6-phosphate
Formula:C6H13NO8P
KEGG:
C00352
+
h2o
Name:H2O
Formula:H2O
KEGG:
C00001
<==>
f6p
Name:D-Fructose 6-phosphate
Formula:C6H11O9P
KEGG:
C00085
+
nh4
Name:Ammonium
Formula:H4N
KEGG:
C01342
Classification: EC:3.5.99.6
|
| Ligand Information | | Ligands | | | Metals | | | |
Protein Summary
The TM0813 gene from Thermotoga maritima encodes a glucosamine-6-phosphate deaminase (PF01380, COG0449, EC 3.5.99.6). The TM0813 structure adopts a SIS (Sugar ISomerase) domain fold and shows strong similarity (main-chain rmsd of 1.8 Å over 291 residues with a sequence identity of 32%) with a glucosamine-6-phosphate deaminase from two other hyperthermophiles, Pyrococcus horikoshii (PDB ids: 2e5f, 2df8, 2dec) and Pyrococcus furiosus (2cb0) (Kim 2007). Similar results were obtained for a homolog from Listeria monocytogenes (PDB id: 2aml).
[SIS domain fold family contains 3 children: mono-SIS, double-SIS and phosphoglucose isomerase (PGI) SIS. TM0813 is part of the double-SIS group along with 1wiw, 1tzb, 1tzc, 1x9i, 1x9h and others. Look at fold vs function variants]
[Compare active site residues with 2cb0]
Ligand Summary
References
