1j5p

Protein Summary

 The gene TM1643 from Thermotoga maritima encodes an enzyme aspartate dehydrogenase EC:1.4.1.21 COG:COG0136.  The structure of this enzyme has been previously solved PDB:1H2H [Ref].  TM1643 is found in an operon with two other genes that encode enzymes involved in the biosynthesis of NAD.  The structure, 1j5p, contains a N-terminal Rossmann fold domain with a bound NAD(+) cofactor and a C-terminal alpha+beta DUF108 (SCOP). The active site of the enzyme is located at the interface between the two domains.  The enzyme is highly specific for aspartate and does not oxidize the similar amino acids glutamate and asparagine.  Other bacteria use oxidation of glutamate and asparagine as the first steps of de novo NAD biosynthesis.  In contrast, Thermotoga maritima uses NAD as a cofactor in the first step towards the synthesis of NAD ( PSI-SGKB).

Ligand Summary

References